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Society for Behavioral Neuroendocrinology |
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| 2003 Meeting Workshop |
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Hosted by the University of Cincinnati Cincinnati, Ohio June 25 - June 29, 2003 ... WORKSHOP INFORMATION (Updated ) An premeeting workshop on the cutting-edge topic of "Functional Genomics in Behavioral Neuroendocrinology" will take place on the afternoon of Wednesday June 25th. Functional genomics, in particular micro array, has been proven to be a useful tool for analysis of differential gene expression in many areas of neuroscience, including drug addiction, schizophrenia, and circadian rhythms. Recently, micro array technology has been employed to study changes in gene expression related to behaviors or neuroendocrine events. This workshop will present some of the latest findings regarding use of micro array in (behavioral) neuroendocrinology. Moreover, the workshop will include discussions on topics concerning methodology, data analysis, and confirmation. This workshop is being co-organized by Lique Coolen (University of Cincinnati) and Jessica Mong (Rockefeller University). The workshop will take place in the Vontz Center for Molecular Studies, immediately adjacent to the Kingsgate Conference Center (refreshments and snacks will be provided). It will allow an opportunity for members of the SBN who are interested in this new upcoming technology to interact with outside speakers, each other, and with local members of the University of Cincinnati Genomics Core facilities. Directions to the Vontz: You can not miss it! It's the funky looking building next to the Kingsgate, on the corner of Martin Luther King Boulevard and Eden Avenue. Enter via the main entrance on Eden Avenue. The auditorium is immediately to your left upon entering the building. Registration: Registration for this workshop is free. If you are interested in participating, please email Lique Coolen by June 20. Preregistration is encouraged, as we need to order refreshments. TENTATIVE PROGRAM 12.00-1.30
Sensitivity and Performance of Microarray Technology in Neuronal
Tissue
Fishing for Function: Using cDNA Microarrays to Generate Hypotheses
cDNA microarrays are useful descriptive tools, but they have clear limitations.
A priori logical frameworks can facilitate the filtering of microarray
data; follow-up (confirmatory) experiments are becoming the norm. In the
effort to connect descriptive changes in gene expression to functional
(causal) explanations, the generation of testable hypotheses is a critical
first step. Data describing changes in hypothalamic gene expression during
the hamster seasonal reproductive cycle will be discussed as one example
of linking a serendipitous discovery of changes in gene expression to organismal-level
function.
Ovarian Steroid-Dependent Modulation of Hippocampal Function: Clues
from a Proteomic-Genomic Analysis
Ovarian steroids have been shown to modulate various aspects of hippocampal
structure and function. Of particular interest are observations that estradiol
influences synapse turnover and the induction of synaptic plasticity in
the CA1 region. We have taken a proteomic/genomic approach to identify
participating gene transcripts. I will describe our approach, some of its
advantages and disadvantages, and some results from our initial and follow-up
studies.
1.30-1.45 Coffee Break
1.45- 3.15
Creation and Mining of a Gene Expression Database to Define Genes,
Pathways, and Regulatory Mechanisms in the Nervous System
This talk will cover Technical, Biological, and Analytical Considerations
for MicroArray Experiments and will show the application of a variety of
analysis approaches that allow for gene and functional gene group discovery
in the context of tissue specific gene expression data.
Delivering the Genome with Real-Time PCR: Streamlining Studies in
Functional Genomics
Overview and application of Real-Time PCR in gene expression studies
and the complementary relationship between microarrays and
Modeling early neural differentiation: Converging evidence from the
transcriptome and proteome
I propose to talk about a viral-transformed neural precursor system
that we developed to study the control of neural cell fate. I would discuss
the implications of both the convergence and the divergence of the mRNA
and protein data.
3.15-3.30 Coffee Break
3.30-4.30
Viral Vector Mediated Gene Transfer in Behavioral Research
Viral vector mediated gene transfer is an efficient means of manipulating
behaviorally relevant gene expression in a region-specific manner in adult
animals. I will discuss methodological considerations in vector construction
and experimental design for using this technology in behavioral studies.
RNA interference: new tool for neuroscience research
4.30-5.00
Affymetrix GeneChip Technology-the 10K Mapping GeneChip
An overview of the latest developments in DNA Analysis utilizing the
Affymetrix GeneChip Technology-the 10K Mapping GeneChip designed for High
Density SNP Genotyping in Linkage Analysis, and CustomSeq GeneChips designed
to allow rapid and highly accurate Re-sequencing Applications.
5.00-5.30
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